A few days ago, the "various abalone calmodulin cDNA sequence" invented by the South China Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences Jiang Jingzhe, Wang Jiangyong and others was granted a national invention patent.
The invention relates to the field of molecular biology and functional gene application. The disclosed calmodulin gene is first discovered in abalone. The invention uses the heterogenous abalone as a material, and specific primers are designed according to the identified partial gene sequences to obtain the abbreviated calvarial calmodulin gene sequence by PCR amplification, which has a conserved structural domain and active center of calmodulin, and four of them. The conserved domains are located in the 16-36, 45-72 and 84-109 and 118-145 regions of the protein sequence respectively; the active sites of the first conserved domain include: Asp-20, Asp-22, Asn-24 and Glu- 31; The second conserved domain contains Asp-56, Asn-58, Asn-60, and Glu-67; the third conserved domain contains Asp-93, Asp-95, Ser- 97 and Glu-104; the active site of the fourth conserved domain includes: Asp-129, Asn-131, Asp-133 and Glu-140.
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